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KMID : 0357819960200010024
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Korean Journal of Legal Medicine
1996 Volume.20 No. 1 p.24 ~ p.36
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Genetic Diversities of Three Short Tandem Repeats(STRs) Loci in Korean Amplified by Multiplex PCR
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권국환/Kwon, Kuk-Hwan
이희석/이혜린/황적준/박종진/송은섭/Lee, Hee-Suk/Lee, Hye-Rin/Hwang, Juck-Joon/Park, Chong-Jin/Song, Eun-Sub
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Abstract
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The tetrameric STRs loci were studied in a population of Korean(n = 223) for allele frequency distribution and applicability to identity and paternity testing, using multiplex PCR with electrophoresis of the PCR products in DNA sequencing gels and subsequent detection of allelic fragments by silver staining. The STRs loci analyzed were HUMCSFIPO, HUMTPDX, and HUMTHOI : in the Korean population, 9 alleles with their frequency range of 0.002-0.363 are detected in the HUMCSFIPO, 5 alleles with those of 0.020-1.480 in the HUMTPDX, and 6 alleles with those of 0.002-0.489 in the HUMTHO1. The highest observed heterozygosity is found at the locus HUMCSFIPO(0.709), those of the loci HUMTPDX and HUMTHOI being 0.646 and 0.632,
respectively. All loci meet Hardy-Weinberg expectations : there are good agreement between the observed and expected values under Hardy-Weinberg equilibrium. Moreover, pairwise comparisons between loci show allelic independence for all the 3 loci comparisons.
The power of discrimination(PD) determined for the locus HUMCSFIPO is 0.8896 (88.96%), that for the HUMTPDX is 0.8818(88.18%), and that for the HUMTHOI is 0.8367(83.67%) ; the combined power of discrimination for the triplex is 0.9976(99. 76%). The power of exclusion(PEX) calculated for the loci HUMCSFIPO, HUMTPDX, and HUMTHOI, being prior probability that a falsely accused father will be excluded, are 0.5173(51.73%), 0.4610(46.10%), and 0.5101(51.01%), respectively, and the cummulative power of exclusion(CPE) for all the three loci is 0.8726(87.26%).
Thus, these allelic frequency data can be used to construct the database of the multiplex PCR-based DNA profile in the Korean population. The calculated parameter, "power of discrimination (PD) " and "power of exclusion (PEX)", show the informativeness of these loci for the determination of identity and relatedness of individuals.
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KEYWORD
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